Overview

This technical note explains how controlled‑flow and zero‑flow capillaries used in capillary electrophoresis (CE) behave across different pH conditions. These capillaries are engineered to deliver consistent flow rates regardless of pH, eliminating flow variability that could otherwise affect migration times or separation reproducibility. Because the capillary design remains unaffected by pH changes, users are free to adjust pH based solely on analytical needs—specifically, the pH that delivers the best separation, resolution, and peak shape for their analytes. .

This simplifies method development by removing flow‑related pH concerns.

Optimized pH with Zero‑Flow and Controlled‑Flow Capillaries

Zero‑flow and controlled‑flow capillaries are designed to be pH independent, meaning their flow characteristics remain consistent across a wide range of pH conditions. Changes in buffer pH do not significantly alter the flow rate within these capillaries.

Because of this stability, the optimal pH for your application is simply the pH that provides the best separation for your analytes. You can select the pH based entirely on analytical performance without needing to account for flow variability.

Click HERE to view ordering information and images for Zero‑Flow and Controlled‑Flow capillaries.